In this era, mobile phone is one of the significant technological advancement. These devices help individuals share information and stay connected with each other. The purpose of this study is to find out the microbiological contamination on mobile phones and determine the effectiveness of self-prepared antimicrobial solution. Hence a total of 30 mobile phones were randomly collected and analyzed with positive and negative controls for microbial contamination which is compared after application of solution. The results show a decrease of 81.40% after the application of antimicrobial solution in SDA. While in NA the average number has been decreased by 59.87%. Testing with ethanol as disinfecting agents for mobile phones significantly reduced the number of microbes on the surface of mobile phones. It is important to be aware about the reduction of microbes (both fungi and bacteria). Based on the study it was evident to conclude that the formulation is effectively inhibiting the growth of microorganisms found on the surface of mobile phones. Hence, it was suggested to the participant to follow and adhere to the simple hygiene measures by using the formulation for a healthy lifestyle.