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Development and Validation of and screening test for antibiotics detection in manure

Currently, one of the main concerns of regulatory authorities is the presence of chemical contaminants in food and the need to establish appropriate
programs to monitor this risk, safeguarding consumers. Among these, antibiotic residues are of great importance in food production and public
health, due to the consequences for human health, such as the development of antimicrobial resistance. As for the presence of antibiotic residues in
husbandry manure, a large percentage of these drugs have been described to be excreted in animal waste, since they are not completely metabolized.
This may vary between 90-30%, with persistence of the microbiological activity. Recently, high concentrations of antibiotics have been reported
by our research group in feces of treated poultry, compared to concentrations found in edible tissues or other non-edible tissues. The detection of
antimicrobials in manure could be used as a non-invasive sampling method and thus as a promising tool for monitoring the use of antimicrobials
in animal production. Also, considering that there are currently no low-cost commercial methods, registered or patented for the rapid detection
of different antimicrobials in non-invasive animal matrices such as feces used in animal husbandry, we proposed to implement and validate a new
screening methodology for the detection of antibiotic residues in manure. This rapid, economical, and non-invasive tool will allow the early detection
of tetracyclines, β-lactams, macrolides, quinolones, sulfonamides and aminoglycosides in feces of production animals during the entire productive
stage and prior to slaughter. The methodology will be verified in incurred samples obtained from experimental animals treated with the antibiotics
of interest with therapeutic doses (in vivo study) and subsequently checked in field samples, obtained from different species of productive animals.


Javiera Cornejo Kelly, Lisette Lapierre A., Betty San Martin N., Hector Hidalgo O.,
Aldo Maddaleno T

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