Dengue is the most common arthropod borne endemo- epidemic arboviral infection in many tropical and sub- tropical regions of the world. An estimated 50-100 million dengue infections occur annually. Case fatality rate vary from 1 to 5% but can be less than 1% with appropriate diagnosis and treatment (WHO dengue) 1. As the laboratory diagnosis is confusing, the study was conducted to evaluate the different laboratory test done in microbiology, biochemistry and pathology. This study was conducted at Princess Esra Hospital, Deccan College of Medical Sciences. Total 1266 cases were studied in the age group of 5 yrs to 80 yrs (both children and adult) from July 2018 to December 2018. During this 6 month of study the total numbers of positive cases were 385 and negative cases were 881. The laboratory tests which were performed were NS1 Antigen, Dengue IgM, Dengue IgG, CBP and Platelet count, Prothrombin Time, INR, Activated partial Thromboplastin Time, Alanine Transaminase, Aspartate Transminase.